My research interests are in the areas of biophysics, atomic force microscopy, nanomechanics, and hemostasis. Specific interests include:
For more information on research of teaching see my personal website.
Helms, Christine C., Shannon Kapadia, Anne C. Gilmore, Zhexi Lu, Swati Basu, and Daniel B. Kim-Shapiro. "Exposure of fibrinogen and thrombin to nitric oxide donor ProliNONOate affects fibrin clot properties." Blood Coagulation & Fibrinolysis: An International Journal in Haemostasis and Thrombosis (October 13, 2016). doi: 10.1097/MBC.0000000000000602
W. Li, J. Sigley, M. Pieters, C.C. Helms, C. Nagaswami, J. W. Weisel, M. Guthold “Fibrin Fiber Stiffness Is Strongly Affected by Fiber Diameter, but not by Fibrinogen Glycation.” Biophysical Journal. 2016.110(6):1400-1410.
C. Liu, N. Wajih, X. Liu, S. Basu, J. Janes, M. Marvel, C. Keggi, C.C. Helms, A.N. Lee, A.M. Belanger, D.I. Diz, P.J. Laurienti, D.L. Caudell, J. Wang, M.T. Gladwin, D.B. Kim-Shapiro. “Mechanisms of Human Erythrocytic Bioactivation of Nitrite.” Journal of Biological Chemistry. 2015.290(2):1281-94.
M.J. Berry, N.W. Justus, J.I. Hauser, A.H. Case, C.C. Helms, S. Basu, Z. Rogers, M.T. Lewis, G.D. Miller. “Dietary nitrate supplementation improves exercise performance and decreases blood pressure in COPD patients.” Nitric Oxide. 2014.48(1):22-30.
D. Wang, I. Cortes-Puch, J. Sun, S.B. Solomon , T. Kanias, K. Remy, J. Feng, M. Alimchandani, M. Quezado, C.C. Helms, A. Perlegas, M.T. Gladwin, D.B. Kim-Shapiro, H. Klein, C. Natanson. “Transfusions of older stored blood worsens outcomes in canines depending on the presence and severity of pneumonia.” Transfusion. 2014.54(7):1712-1724.
C.C. Helms, M. Marvel, W. Zhao, M. Stahle, R. Vest, G.J. Kato, J.S. Lee, G. Christ, M.T. Gladwin, R.R. Hantgan, D.B. Kim-Shapiro. “Mechanisms of hemolysis-associated platelet activation.” Journal of Thrombosis and Haemostasis. 2013.11(12):2148-2154.
C.C. Helms and D. Kim-Shapiro. “Hemoglobin-mediated nitric oxide signaling.” Free Radic Biol Med. 2013. 61:464-472
D. Wang, B. Piknova, S.B. Solomon, I. Cortes-Puch, S.J. Kern, J. Sun, T. Kanias, M.T. Gladwin, C.C. Helms, D.B. Kim-Shapiro, A.N. Schechter, C. Natanson. “In vivo reduction of cell-free methemoglobin to oxyhemoglobin results in vasoconstriction in canines.” Transfusion. 2013. 53(12):3149-63
R.D. Averett, B. Menn, E.H. Lee, C.C. Helms, T. Baker, M. Guthold. “A modular fibrinogen model that captures the stress-strain behavior of fibrin fibers.” Biophysics Journal. 2012. 103(7):1537-44.
J. Tejero, S. Basu, C.C. Helms, N. Hogg, S.B. King, D.B. Kim-Shapiro, M.T. Gladwin. "Low NO concentration dependence of the reductive nitrosylation reaction of hemoglobin". Journal of Biological Chemistry. 2012. 287(22):18262-74
C.C. Helms, R. Ariëns, S. Uitte de Willige, K. Standeven, M. Guthold. “Alpha-alpha Cross-Links Increase Fibrin Fiber Elasticity and Stiffness.” Biophysical Journal. 2012. 102(1):168-175.
S. Baker, J. Sigley, C. Carlisle, J. Stitzel, J. Berry, M. Guthold. “The mechanical properties of dry, electrospun fibrinogen fibers.” Materials Science and Engineering C. 2011. 32: 215-221
J. Kim, H. Song, I. Park, C. Carlisle, K. Bonin, M. Guthold. “Denaturing of single electrospun fibrinogen fibers studied by deep ultraviolet fluorescence microscopy.” Microscopy Research and Technique. 2011. 74(3): 219-224.
W. Liu, C.R. Carlisle, E.A. Sparks, M. Guthold. “The mechanical properties of single fibrin fibers.” Journal of Thrombosis and Haemostasis. 2010. 8(5): 1030-1036 (co-first author)
C.R. Carlisle, E.A. Sparks, C. Der Loughiam, M. Guthold. “Strength and Failure of Fibrin Fiber Branch Points.” Journal of Thrombosis and Haemostasis. 2010. 8(5): 1135-1138.
C.R. Carlisle, C. Coulais, M. Guthold. “The mechanical stress-strain properties of single electrospun collagen type I nanofibers.” Acta Biomaterialia. 2010. 6(8): 2997-3003.
C.R. Carlisle, C. Coulais, M. Namboothiry, D. Carroll, R.R. Hantgan, M. Guthold. “The mechanical properties of individual, electrospun fibrinogen fibers.” Biomaterials. 2009. 30(6): 1205-1213.
Ph.D., Wake Forest University 2010
B.S., University of Mary Washington 2005
Atomic Force Microscopy